High-throughput DNA sequencing of Klebsiella pneumoniae plasmid pNDM-LJ
10.3969/j.issn.1006-5725.2016.05.006
- VernacularTitle:肺炎克雷伯菌质粒pNDM-LJ的高通量测序分析
- Author:
Ling YANG
;
Hailing TANG
;
Dingqiang CHEN
;
Hairong QIU
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
PNDM-LJ;
BlaNDM-1;
Carbapenemase
- From:
The Journal of Practical Medicine
2016;32(5):706-709
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the resistance of the Klebsiella pneumoni-ae and the Klebsiella pneumoniae plasmid pNDM-LJ carrying blaNDM-1 by high-throughput DNA sequencing. Methods High-throughput DNA sequencing was carried out by the Illumina Miseq platform , and sequencing data were assembled by Edena software. Contigs were annotated by the RAST server and analyzed by the BLAST server. Results The plasmid pNDM-LJ was 54-kb in size with a GC content of 49%. The plasmid encoded 52 putative functional genes and belonged to the IncX3 group in incompatible classifications. Analysis of the plasmid sequence revealed high similarity with other IncX3 plasmids. The blaNDM-1 gene was located in a complicated gene environment possibly constructed by several transposition events. The 5′ and 3′ ends of the blaNDM-1 gene were adjacent to the ISAba125 and IS 26 respectively , forming a 10.8-kb transposon-like structure. Conclusion The plasmid pNDM-LJ carried the blaNDM-1 gene being resistant to carbapenems and played a possibly impor-tant role in transmission of blaNDM-1 in China.
