Effects of phosphocreatine postconditioning on cerebral ischemia-reperfusion injury in rats
- VernacularTitle:磷酸肌酸后处理对大鼠脑缺血-再灌注损伤的影响
- Author:
Xiaofen SUN
;
Fang KANG
;
Yujun SHEN
;
Yuxian SHEN
;
Juan LI
- Publication Type:Journal Article
- Keywords:
Phosphocreatine;
Post-ischemia treatment;
Oxidative stress;
Apoptosis
- From:
The Journal of Clinical Anesthesiology
2016;32(3):268-272
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of phosphocreatine postconditioning on cerebral ischemia-reperfusion(IR)injury in rats.Methods Thirty-six SD rats were randomly divided into three groups:groups Sham,IR (treated with normal saline)and PCr.IR was induced by intraluminal middle cerebral artery occlusion (MCAO).All treatments were given intravenously at the begining of reperfusion.Twenty-four hours after the reperfusion, neurological deficit score and magnetic resonance scan were performed.serum concentrations of malonaldehyde and 4-hydroxynonenal,cere-bral infarct volume and destruction of cerebral cortex were estimated.Neuronal apoptosis was further assessed by immunohistochemistry and immunofluorescent staining of caspase-3 and NeuN. Results Compared with group IR,phosphocreatine significantly decreased neurological deficit score, infarct volume,malonaldehyde and 4-hydroxynonenal levels(P < 0.05 ).Cortex structure was more complete,as well as neuronal apoptotic index was smaller in group PCr (P <0.05).Conclusion PCr can reduce cerebral infarct volume,thereby promote neurofunctional recovery.The mechanism of Pcr is related to reduced oxidative stress and inhibitted apopotosis during IR.
