Effect of small direct-current electrical stimulation on migration and invasion related MMPs/TIMPs expression of trophoblast cells
10.3969/j.issn.1671-8348.2016.07.002
- VernacularTitle:微电场对滋养细胞迁移／侵袭相关MMPs／TIMPs表达的影响
- Author:
Juan ZHANG
;
Mingyong LI
;
Yuan HE
;
Huai BAI
;
Ping FAN
- Publication Type:Journal Article
- Keywords:
trophoblasts cells;
matrix metalloproteinases;
tissue inhibitor of metalloproteinase;
migration;
invasion;
electrical field
- From:
Chongqing Medicine
2016;45(7):869-872
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of small direct‐current electrical stimulation on migration and invasion related MMPs/TIMPs expression of trophoblast cells .Methods The trophoblast cells were exposed to the direct current electrical field at 150 mV/mm for 5 and 10 hours .Cell images were recorded with continuous photographing and analyzed by image analyzer .The ex‐pression levels of MMP2 ,MMP9 ,TIMP1 and TIMP2 were measured using quantitative RT‐PCR and Western blot .Results In non‐electrical field culture trophoblast cells migrated slowly with random directions .Trophoblast cells cultured in media containing 10% calf serum with the application of 150 mV/mm direct current electrical stimulation ,showed marked cathodal migration (P<0 .01) ,the cell body stretched ,perpendicular to the direction of the electric field .Compared with the non‐electrical field stimulation controls ,trophoblasts under the electrical field stimulation had the increased MMP2 mRNA and protein expression (P< 0 .05) , while MMP9 ,TIMP1 and TIMP2 had no obvious changes of mRNA or protein expressions .Conclusion Physiological direct‐cur‐rent electrical fields might induce directed migration and perpendicular orientation of trophoblast cells .The enhanced MMP2 expres‐sion may play an important role in the migration and invasive activity of trophoblast cells in small electrical field .
