Protective Effect of Diclipterachinensis Polysaccharide P2 B on Carbon Tetrachloride-induced Injury of Liv-er Cell Line L-02
- VernacularTitle:狗肝菜多糖P2 B对CCl4致L-02肝细胞损伤的保护作用
- Author:
Danhua XU
;
Yuyuan HE
;
Shihao YANG
;
Kaiqi LI
;
Xionghui YANG
;
Zhibin SHEN
- Publication Type:Journal Article
- Keywords:
Diclipterachinensis polysaccharide;
Hepatocyte injury;
Carbon tetrachloride
- From:
China Pharmacist
2016;19(4):675-677
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the protective effect of Diclipterachinensis polysaccharide P2B on liver cell line L-02 injury in-duced by carbon tetrachloride ( CCl4 ) . Methods:The human liver L-02 cells were cultured, and the injury model was built by CCl4 . The L-02 cells were divided into the normal control group, the CCl4-damaged group, and the P2B sample groups (0. 125, 0. 250 and 0. 500 mg· ml-1 ). The contents of alanine aminotransferase ( ALT), aspartate aminotransferase ( AST), superoxide dismutase (SOD) and malondialdehyde (MDA) were determined by MTT assay. Results:Compared with the CCl4-damaged group, P2B could improve the activity of L-02 cells, and the activity of AST and ALT in the supernatant was significantly reduced, and the content of SOD in the cells was increased and that of MDA was decreased. Conclusion:P2B can significantly prevent L-02 cells from the damage induced by CCl4 in a dose-dependent manner, and the mechanism may be related with the anti-oxidative activity of P2B.
