Content Determination of Extract, Polysaccharide and Mannose in Dendrobii Officinalis Caulis from Lishui
- VernacularTitle:丽水地产铁皮石斛中浸出物、多糖及甘露糖的测定
- Author:
Huali YU
;
Lei FAN
;
Juhua MAO
;
Zhangjin CHEN
- Publication Type:Journal Article
- Keywords:
Dendrobii officinalis Caulis;
Extract;
Polysaccharide;
Mannose
- From:
China Pharmacist
2016;19(4):761-763
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the quality of Dendrobii officinalis Caulis cultivated in Lishui by the content determination of ex-tract, polysaccharide and mannose. Methods:Totally 26 batches of Dendrobii officinalis Caulis were dried at 55℃, and the ethanol ex-tract, polysaccharide and mannose was determined respectively by hot dipping, phenol-sulphuric acid colorimetry and pre-column deri-vatization HPLC method according to the determination methods for Dendrobii officinalis Caulis recorded in Chinese Pharmacopoeia (2010 edition). Results:The contents of extract, polysaccharide and mannose in Dendrobii officinalis Caulis during the collection pe-riod were higher than those of the pharmacopoeia standard, and there were significant differences among the batches. Conclusion:The test can provide theory basis for the quality evaluation of Dendrobii officinalis Caulis cultivated in Lishui, and provide guidance for the planting of the herb.