Simultaneous Determination of Paeoniflorin,Hesperidine,Baicalin and Chrysophanol in Xiaoshi Lidan Cap-sules by Dual Wavelength HPLC
- VernacularTitle:HPLC双波长法同时测定消石利胆胶囊中芍药苷、橙皮苷、黄芩苷和大黄酚的含量
- Author:
Yantao SUN
- Publication Type:Journal Article
- Keywords:
Xiaoshi Lidan capsules;
HPLC;
Paeoniflorin;
Hesperidine;
Baicalin;
Chrysophanol;
Content determination
- From:
China Pharmacist
2016;19(4):801-803
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for the simultaneous determination of paeoniflorin, hesperidine, baicalin and chry-sophanol in Xiaoshi Lidan capsules by dual wavelength HPLC. Methods:An Inertsil ODS-3 C18 column (150 mm × 4. 6 mm,5 μm) was used. The mobile phase was acetonitrile-0. 2% phosphoric acid with gradient elution. The flow rate was 1. 0 ml·min-1 , the de-tection wavelength was 230nm for paeoniflorin, hesperidine and baicalin, and 280nm for hrysophanol, and the column temperature was 40℃. Results:The linear range of paeoniflorin, hesperidine,baicalin and chrysophanol was 0. 030-0. 592 μg(r=0. 999 9), 0. 063-1. 264 μg(r=1. 000 0), 0. 205-4. 094 μg(r=1. 000 0) and 0. 008 2-0. 164 μg(r=1. 000 0), respectively. The average recovery of the four components was 99.49% (RSD =0.85%), 99.74%(RSD =0.71%), 99.75%(RSD =0.47%) and 99.08%(RSD =1. 28%), respectively. Conclusion:The method is simple and accurate, which can be used for the content determination of paeoniflor-in,hesperidine,baicalin and chrysophanol in Xiaoshi Lidan capsules.
