Study on Preparation of Immobilized Thrombin Treated with Silk Fibroin
- VernacularTitle:丝素固定化凝血酶的工艺研究
- Author:
Yanli MIAO
;
Guangren YU
;
Yinlong XU
;
Yifeng LIU
;
Weifeng HUANG
;
Shaohong CHEN
- Publication Type:Journal Article
- Keywords:
Silk fibroin;
Thrombin;
Immobilization;
Activity yield
- From:
China Pharmacist
2016;19(4):671-674
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of silk fibroin on the immobilization of thrombin. Methods: The immobilized thrombin was prepared using silk fibroin as the carrier and glutaraldehyde as the crosslinking agent. With activity yield as the index, the process conditions of silk fibroin immobilized thrombin were determined by an orthogonal test. Results:The optimum process con-ditions of immobilized thrombin treated with silk fibroin were as follows:the immobilization time was 6 h, the enzyme dosage was 2 400 NIH·g-1 casein, the temperature was 25℃ and pH was 7. 6. The activity recovery of immobilized thrombin was 67. 22%. Conclu-sion:Silk fibroin has the positive immobilization effect on thrombin.
