Determination of Total Ginkgo Flavonoid in Self-emulsifying Drug Delivery System by HPLC
- VernacularTitle:HPLC测定银杏总黄酮自乳化微乳中银杏总黄酮的含量
- Author:
Xiangru LIAO
;
Song FANG
;
Qiongzhi SHI
;
Ying CHEN
- Publication Type:Journal Article
- Keywords:
Total ginkgo flavonoid;
Self-emulsifying drug delivery system;
Determination;
HPLC
- From:
China Pharmacist
2016;19(4):764-766
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop an HPLC method for determining the total ginkgo flavonoid in self-emulsifying drug delivery sys-tem. Methods:Effective chromatographic separation was achieved using a phenomenex C18 column (250 mm × 4. 6 mm, 5 μm) with a mobile phase composed of methanol and water (0.4% phosphoric acid) with the ratio of 50 ∶50 (v/v). The mobile phase was pumped using an isocratic HPLC system at a flow rate of 1. 0 ml·min-1 , the detection wavelength was 360 nm and the column temper-ature was 30 ℃. Results:The three components in the total ginkgo flavonoid were well separated by the proposed method. The linear relationship between the peak area and the concentration was promising within the range of 2. 0-40. 0 μg·ml-1 for quercetin, 3. 0-60. 0 μg·ml-1 for kaempferide and 2. 0-40. 0 μg·ml-1 for isorhamnetin. The mean recovery of quercetin, kaempferide and isorham-netin was 98. 4%, 99. 7% and 100. 5% with RSD of 0. 92%,0. 62% and 1. 24% (n=9), respectively. Conclusion:The method is specific and stable in the determination of total ginkgo flavonoid in self-emulsifying drug delivery system.
