Determination of Baicalin and Geniposide in Ganxueguanliu Huoxue Capsules by HPLC
- VernacularTitle:HPLC法同时测定肝血管瘤活血胶囊中栀子苷和黄芩苷的含量
- Author:
Chunjing FENG
;
Jianing HOU
;
Guiyang LIU
;
Xiang LI
;
Chuntong LI
- Publication Type:Journal Article
- Keywords:
Ganxueguanliu Huoxue capsules;
Baicalin;
Geniposide;
HPLC;
Content determination
- From:
China Pharmacist
2016;19(4):772-774
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To build an HPLC method for the determination of baicalin and geniposide in Ganxueguanliu Huoxue cap-sules. Methods:An Agilent Eclipse XDB-C18 (150 mm × 4. 6 mm, 5μm) column was chosen for the analysis. The mobile phase con-sisted of 0. 2% phosphoric acid (A) and methanol (B) with gradient elution. The flow rate was 1. 0 ml·min-1. The detection wave-length was set at 238 nm and the column temperature was 25℃. Results:A good linear relationship was observed within the range of 0. 418-8. 360 μg·ml-1 for baicalin (r=0. 999 9) and 0. 226-4. 524μg·ml-1 for geniposide (r=0. 999 9). The average recovery of baicalin and geniposide was 99. 79% (RSD=1. 55%, n=6) and 100. 2%(RSD=2. 05%, n=6), respectively. Conclusion: The method is rapid, convenient and specific, which can be used for the content determination of baicalin and geniposide in Ganxueguanliu Huoxue capsules.