Simultaneous Determination of 5 Active Ingredients in Begonia Grandis Dry from Different Habitats by UP-LC
- VernacularTitle:UPLC法测定不同产地秋海棠中5种药效成分的含量
- Author:
Hui LIU
;
Hua LI
- Publication Type:Journal Article
- Keywords:
Begonia grandis Dry;
Different habitates;
UPLC;
Content determination
- From:
China Pharmacist
2016;19(4):780-782
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of protocatechuic acid, protocatechuic, catechin, epicatechin and rutin in Begonia grandis Dry. Methods:The 5 active ingredients in the samples were extracted by 70% methanol and analyzed by ultra performance liquid chromatography (UPLC). Chromatographic separation was performed on a Waters ACQUITY HSS T3(2. 1 mm × 100 mm, 1. 8 μm) column with gradient elution, and the mobile phase was solvent A: acetonitrile and solvent B:0. 2% glacial acetic acid (0-12 min:A:5→25 and B:95→75;12-30 min:A:25→50 and B:75→45). The effluent was monitored by a VWD detector set at 280 nm. The flow rate was set at 0. 2ml/min and the injection volume was 1μl. The column temperature was maintained at 30℃. Results:The calibration curves were all linear (r≥0. 99) over the test ranges. The average recovery was 96. 92%-99. 75%(n=6). Conclusion:The established method can rapidly obtain an accurate and reproducible result, which can be used to control the quality of Begonia grandis Dry.
