Simultaneous Determination of Flavonoids and Phenolic Acids in Herba Gnaphalium Affinis by HPLC
- VernacularTitle:HPLC法同时测定佛耳草中黄酮类和酚酸类成分的含量
- Author:
Yiqing MAO
- Publication Type:Journal Article
- Keywords:
HPLC;
Herba Gnaphalium Affinis;
Chlorogenic acidrutin;
3,5-O-Dicaffeoyl quinic acid;
Quercetin;
Luteolin
- From:
China Pharmacist
2016;19(4):783-785
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop an HPLC method for the simultaneous determination of flavonoids and phenolic acids in Herba Gnaphalium Affinis. Methods:The analysis was carried out on a Diamonsil C18 column (200 mm × 4. 6 mm, 5μm) with gradient elu-tion using methanol-0. 4% phosphoric acid as the mobile phase. The flow rate was 1. 0 ml·min-1 and the detection wavelength was 327 nm for flavonoids and 360nm for phenolic acids,the column temperature was 30℃, and the sample size was 10 μl. Results:The calibration curves were linear over the range of 0. 041-0. 821 μg for chlorogenic acidrutin, 0. 127-2. 549 μg for 3, 5-O-dicaffeoyl quin-ic acid, 0. 020-0. 411 μg for quercetin and 0. 040-0. 806μg for luteolin. The average recovery was 98. 31% ( RSD = 1. 19%), 97. 51%(RSD=1. 52%), 98. 29%(RSD=1. 22%) and 98. 73%(RSD=1. 47%), respectively. Conclusion:The described meth-od is simple and reproducible, which can be used for the quality control of Herba Gnaphalium Affinis.
