Immunofluorescence probe based on fluorescent carbon dots and its application to the special recognition of Escherichia coli

VernacularTitle:基于荧光碳点的免疫荧光探针构建及其对大肠杆菌的特异性识别
Author: Wangyuan CHE ; Kun YANG ; Fan LI ; Changjun LIU ; Feng TIAN
Publication Type:Journal Article
Keywords: fluorescent antibody technique; immunofluorescent probes; carbon dots; specificity; Escherichia coli O157∶H7; nanostructures
From: Military Medical Sciences 2016;40(3):202-206
CountryChina
Language:Chinese
Abstract: Objective Carbon dots (CDs) are an emerging carbon nano-material which is environmentally-friendly, economical , efficient and stable .Their fluorescence properties can match the semiconductor quantum dot .Moreover , CDs have more excellent biocompatibilities .The purpose of this experiment is to apply CDs to the fluorescent immune probe to make them a new label , which can replace the traditional fluorescent dyes .Methods Using microwave heating method , the high strength fluorescent carbon dots were prepared .Wtih the EDC coupling method , the high strength fluorescent car-bon dots could bond with Escherichia coli antibodies to form a complex immune fluorescent probe .Specific recognition exper-iments were carried out in the model of E.coli O157∶H7.Results CDs were successfully applied to immune recognition of E.coli O157∶H7 and multicolor fluorescence was observed .Conclusion CDs can serve as a label of the fluorescent im-mune probe , and are expected to become a new type of low toxicity biosensor with independent intellectual property rights .