Downregulation of Ro60 inhibits migration and invasion of human lung adenocarci-noma A549 cells
10.7644/j.issn.1674-9960.2016.03.012
- VernacularTitle:Ro60表达下调抑制肺腺癌A549细胞迁移和侵袭
- Author:
Wan YAO
;
Jilai LIU
;
Pengda SUN
;
Baolei TIAN
;
Bin LIU
;
Yi SONG
;
Zhixian SUN
- Publication Type:Journal Article
- Keywords:
Ro60;
lung adenocarcinoma;
migration;
invasion
- From:
Military Medical Sciences
2016;40(3):213-216
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Ro60 on migration and invasion of lung adenocarcinoma A 549 cells. Methods We knocked down Ro60 and analyzed the ability of migration and invasion of A 549 cells.Quantitative real time ( RT)-PCR and Western blotting were performed to detect mRNA and protein levels of selected molecules associated with migration and invasion of neoplasm .Results When Ro60 was downregulated , the migration and invasion of A 549 cells decreased markedly.Meanwhile,the mRNA expression of matrix metalloproteinase (MMP)9,c-Src etc was observably reduced.Furthermore, downregulation of Ro60 diminished the expression of Src protein and the activation of MMP-9 protein.Conclusion Downregulation of Ro60 inhibits migration and invasion of A549 cells by regulating Src protein and activating MMP-9 protein.
