Quantitative analysis of intestinal bacteria in Caenorhabditis elegans using qPCR

VernacularTitle:基于实时定量PCR技术的秀丽隐杆线虫肠道食物菌鉴定方法
Author: Zhoulong YU ; Yan GAO ; Chenggang ZHANG
Publication Type:Journal Article
Keywords: Caenorhabditis elegans; real-time quantitative polymerase chain reaction (qPCR); commensal gut flora
From: Military Medical Sciences 2016;40(3):248-251
CountryChina
Language:Chinese
Abstract: Objective To establish a method for detecting intestinal bacteria in Caenorhabditis elegans.Methods The gut flora number of 1, 20 or, 50 C.elegans was quantified and compared using real-time quantitative PCR (qPCR). Results The gut flora of a single C.elegans could be detected using qPCR method , which could also reflect the difference in the number of gut bacteria between different samples .Conclusion The qPCR method can be uased to accurately quantify intestinal bacteria even in only one C.elegans and has the advantages of low-cost, high-sensitivity and good-specificity.