Digital PCR analysis of plasma cell-free DNA for non-invasive detective of TKI targeted EGFR mutation in NSCLC patients
10.3760/cma.j.issn.1009-9158.2016.03.003
- VernacularTitle:数字PCR技术在NSCLC患者EGFR-TKI靶向治疗检测中的应用
- Author:
Qian YU
;
Wei GUO
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction;
Lung neoplasms;
Receptor,epidermal growth factor;
Receptor protein-tyrosine kinases;
Molecular tareted therapy;
DNA,neoplasm
- From:
Chinese Journal of Laboratory Medicine
2016;39(3):161-164
- CountryChina
- Language:Chinese
-
Abstract:
It is important to analyze the epidermal growth factor receptor ( EGFR) mutation before makingstrategyonnon-small cell lung cancer ( NSCLC) patients scheduled to EGFR tyrosine kinase inhibitor ( TKI) therapy .Digital PCR is a new generation of molecular diagnostic technique that provides ultra-highersensitive, specific and absolute nucleic acid quantification based on its unique principle.The application of digital PCR indetecting circulate tumor DNA can be the truly tumorliquid biopsy, helps to acquire the accurate EGFR mutation status from peripheral blood and screen out the most appropriate patients for TKI therapy.This breakthrough technology will also contribute to tumor surveillance and drug resistance monitoring.
