Detecting JAK2 V617F mutation in myeloproliferative neoplasms by microarray based digital PCR
10.3760/cma.j.issn.1009-9158.2016.03.007
- VernacularTitle:采用微阵列数字PCR检测JAK2基因V617F突变
- Author:
Xiao XU
;
Qunfeng ZHANG
;
Xinju ZHANG
;
Yigui TANG
;
Huimin REN
;
Rui YANG
;
Ni FAN
;
Bobin CHEN
;
Ming GUAN
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction;
Microchip analytical procedures;
DNA mutational analysis;
Bone marrow neoplasms
- From:
Chinese Journal of Laboratory Medicine
2016;39(3):176-180
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the sensitivity, repeatability and accuracy of microarray digital PCR system in detecting JAK2 V617F mutation, which was closely related to myeloproliferative neoplasms (MPN).Methods All of the 31 MPN patients with JAK2 V617F mutation, including 18 cases of polycythemia vera(PVs),11 primary thrombocythemias (ETs) and 2 primary myelofibrosis (PMFs), were collected from Huashan Hospital, Fudan University during 2014 -2015, while 10 normal controls and 6 cases with abnormal increased hemoglobin were involved.Human erythroleukemia cell line ( HEL ) and colorectal cancer cell SW480 were used as the mutant and the wild type control, respectively.The sensitivity of microarray digital PCR were verified by detecting the gradient diluted mutation standard harboring 30%, 10%, 1%, 0.1%and 0.01%mutant allele burden, respectively .Repeatability was evaluated by detecting 1%and 10% mutated samples for 5 times, respectively.MGB probe real time PCR was selected as the reference method to verify the accuracy of the digital PCR.Results With digital PCR, the accurate quantitation of JAK2 V617F mutation was achieved down to 0.1%, which is approximate to 0.16 copies per microliter.The results obtained from the two kinds of technique showed a high correlation by linear regression analysis (R2 =0.998 3).The results of repeated samples showed CVs as 17.18% for 1%mutant allele burden and 7.50%for 10%.Among all cases, the 31 patients known mutated were detected as positive and 10 controls as negative by both digital PCR and Real time PCR.In another 6 cases, 2 were found JAK2 V617F mutation of low allele burdens of 0.37% and 0.18% by digital PCR but detected as negative by real time PCR.Conclusions Microarray digital PCR offers a higher sensitivity and better repeatability than real time PCR which could help detect rare JAK2 V617F mutations in MPNs accurately.
