Role of JAK/STAT signaling pathway in high glucose-induced damage in human umbilical vein endothelial cells
10.3969/j.issn.1000-4718.2016.03.002
- VernacularTitle:JAK／STAT 信号通路在高糖诱导人脐静脉内皮细胞损伤中的作用
- Author:
Jingqiu LIAO
;
Jiaqiong LIN
;
Weijie ZHANG
;
Lin XU
;
Ximei ZHI
;
Kai LIN
;
Wen WU
- Publication Type:Journal Article
- Keywords:
JAK/STAT signaling pathway;
High glucose;
Human umbilical vein endothelial cells;
AG490
- From:
Chinese Journal of Pathophysiology
2016;32(3):392-397
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate whether Janus kinase/signal transducer and activator of transcription ( JAK/STAT) signaling pathway mediates high glucose-induced damage in human umbilical vein endothelial cells ( HUVECs ) . METHODS:The cell viability was examined by CCK-8 assay.The expression levels of JAK2, STAT3, caspase-9 and en-dothelial nitric oxide synthase ( eNOS) were detected by Western blot .The intracellular levels of reactive oxygen species ( ROS) were tested by DCFH-DA staining followed by photofluorography .Mitochondrial membrane potential ( MMP) was measured by rhodamine 123 staining followed by photofluorography .RESULTS:Treatment of HUVECs with high glucose (40 mmol/L glucose) for 6~12 h enhanced the protein level of phosphorylated JAK 2, peaking at 9 h.Treatment of the cells with high glucose for 6~12 h also increased the protein level of p-STAT3 with the peak value at 12 h.Pretreatment with the inhibitor of JAK/STAT pathway AG490 for 1 h before exposure of the HUVECs to high glucose significantly inhibi-ted the high glucose -induced injury , as evidenced by an increase in the cell viability , decreases in the expression of caspase-9 and the intracellular ROS production , and increases in MMP and the expression of eNOS .CONCLUSION:JAK/STAT signaling pathway is involved in the high glucose-induced damage in HUVECs .
