The correlation between urinary albumin excretion rate and the level of serum miR-130b in patients with type 2 diabetes mellitus
10.3760/cma.j.issn.1000-6699.2016.03.009
- VernacularTitle:2型糖尿病患者血清miR-130 b水平与尿白蛋白排泄率的相关性研究
- Author:
Chuan LYU
;
Li LIANG
;
Ying SHAO
;
Can WU
;
Qiuyue WANG
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus,type 2;
Serum miR-130b;
Diabetic nephropathy
- From:
Chinese Journal of Endocrinology and Metabolism
2016;32(3):220-223
- CountryChina
- Language:Chinese
-
Abstract:
[Summary] The aim of this study was to detect the levels of serum miR-130b expression in patients with type 2 diabetes mellitus and to analyze their correlation with diabetic renal damage. 243 patients with type 2 diabetes mellitus were divided into three groups according to urinary albumin/creatinine ratio ( UACR ): normoalbuminuria group (UACR<30 mg/g, n=103), microalbuminuria group (UACR 30-300 mg/g, n=86), and macroalbuminuria group(UACR>300 mg/g, n=54). The levels of serum miR-130b were validated by realtime polymerase chain reaction. Serum transforming growth factor-β1 (TGF-β1) and tumor necrosis factor-α(TNF-α) were determined by enzyme-linked immunosorbent assay ( ELISA) in all patients and 59 healthy volunteers. Compared with control group, the level of serum miR-130b in the type 2 diabetes mellitus group were significantly decreased, gradually with the increases of UACR. The level of serum miR-130b was inversely correlated with blood urea nitrogen ( r=-0. 295, P<0.05), serum creatinine(r=-0. 316, P<0. 05), UACR(r=-0. 463, P<0. 05), but positively related to the estimated glomerular filtration rate(r=0. 367, P<0. 01). The level of serum miR-130b was also negatively correlated to homeostasis model assessment for insulin resistance, triglyceride, low density lipoprotein-cholesterol, TNF-α, and TGF-β1 (r=-0. 257,-0. 345,-0. 242,-0. 562,-0. 622, all P<0. 01). The present study indicates that serum miR-130b might be a potential new biomarker for early diagnosis of diabetic nephropathy. Serum miR-130b might be involved in the pathogenesis of diabetic nephropathy.
