Expression of autophagy related factors LC3 and Bnip-3 and apoptosis related factors Bcl-2 and Bax in brain tissue of experimental diabetic rats
10.7644/j.issn.1674-9960.2016.03.016
- VernacularTitle:自噬相关因子 LC3、Bnip-3和凋亡相关因子Bcl-2、Bax在实验性糖尿病大鼠脑组织中的表达
- Author:
Fan GAO
;
Jin HU
;
Xiaoli ZHANG
;
Hong JIAO
- Publication Type:Journal Article
- Keywords:
diabetes;
brain tissue;
LC3;
Bnip-3;
Bcl-2;
Bax
- From:
Military Medical Sciences
2016;40(3):230-233
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of autophagy-related factors LC3 and Bnip-3 and apoptosis related factors Bcl-2 and Bax on brain damage in experimental diabetic rats .Methods Thirty SD rats were randomly divided into diabetic group and control group .The diabetic group was injected with 1% streptozotocin ( 60 mg/kg body mass ) and the control group with citrate buffer .The rats were sacrificed after 12 weeks feeding and brain tissues were obtained .Pathological chan-ges were observed and the expression of LC 3, Bnip-3, Bcl-2 and Bax in brain tissues of the rats was detected by immuno-histochemical SP method .Results Compared with the control group ,the diabetic rat brain pathology showed that the cell arrangment was more disorderly and distributed more unevenly , the cell body was smaller , cytoplasm was lighter red , and the number of nerve cells of normal morphology was smaller .The positive number of LC3, Bnip-3 and Bax in brain tissues of diabetic rats was significantly larger than in the control group ,and the difference was statistically significant (P<0.05). However ,the positive number of Bcl-2 was significantly smaller than in the control group ,and the difference was statistically significant (P<0.001).In diabetic rats, LC3 and Bnip-3 showed a weak positive correlation(P<0.05), Bcl-2 and Bax were irrelevant, Bnip-3 and Bax were positively weakly-correlated(P<0.05),Bcl-2 was not correlated;and Bcl-2 and Bax were irrelevant.Conclusion LC3,Bnip-3 and Bax in the brain tissue of diabetic rats are overexpressed , while Bcl-2 shows weak expression , indicating that autophagy factors and apoptotic factors are involved in the process of brain injury in diabet -ic rats, which may be one of the mechanisms of brain tissue damage .
