The effects of liraglutide on the proliferation and differentiation of mouse pre-osteoblasts MC3T3-E1
10.3760/cma.j.issn.1000-6699.2016.03.012
- VernacularTitle:利拉鲁肽在高糖环境下对体外培养的小鼠前成骨细胞MC3 T3-E1增殖、分化的影响
- Author:
Jun LI
;
Shuang JIN
;
Juan ZHANG
;
Wei ZHANG
;
Haofeng ZHOU
;
Huijuan ZHANG
- Publication Type:Journal Article
- Keywords:
Glucagon-like peptide-1 analogue;
Liraglutide;
Osteoblast;
Proliferation;
Differentiation
- From:
Chinese Journal of Endocrinology and Metabolism
2016;32(3):232-235
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of liraglutide on the proliferation and differentiation of mouse pre-osteoblasts MC3T3-E1 exposed to higher glucose concentration. Methods MC3T3-E1 cells were cultured and divided into control group and liraglutide group. In liraglutide group, cells were treated with different liraglutide concentrations (10-9 mol/L, 10-8 mol/L, and 10-7 mol/L, respectively) for 48 hours. Cell proliferation was tested with CCK-8. The mRNA expressions of typeⅠcollagen (COL-Ⅰ), osteopontin (OPN), and alkaline phosphase ( ALP) were detected using semi-quantitative RT-PCR. Results ( 1 ) Compared to control group, the proliferation rate of different liraglutide concentration groups (10-9 mol/L, 10-8 mol/L, and 10-7 mol/L) increased significantly (all P<0. 05), the proliferation rate was the highest in 10-8mol/L liraglutide group. (2)The expression of COL-Ⅰ, OPN, and ALP mRNA in liraglutide groups were higher than those in control group (all P<0. 05). The optimal concentration of liraglutide was 10-8 mol/L. Conclusion Liraglutide within a certain concentration range may improve the proliferation and differentiation of mouse pre-osteoblasts MC3T3-E1.
