Research progress in biosynthesis of backbone-cyclized peptides using EPL and PTS
10.7644/j.issn.1674-9960.2016.03.022
- VernacularTitle:EPL和PTS在生物合成主链环肽方面的应用研究进展
- Author:
Bingyu YE
;
Zhihu ZHAO
- Publication Type:Journal Article
- Keywords:
protein splicing;
EPL;
PTS;
peptides biosynthesis
- From:
Military Medical Sciences
2016;40(3):252-255
- CountryChina
- Language:Chinese
-
Abstract:
As internal protein elements , inteins are self-excised from their host protein and catalyze ligation of exteins with a peptide bond .They are widely found in all the three domains of the biological kingdom , and in viral proteins .Intein-mediated protein splicing is a posttranslational autocatalytic process that does not require auxiliary enzymes or cofactors . Protein splicing involves four intramolecular reactions and a small number of key catalytic residues in the intein and exteins . The development of expressed protein ligation ( EPL ) and protein trans-splicing ( PTS ) is assisted by inteins and protein splicing.This review introduces the biosynthesis of backbone-cyclized peptides libraries , and describes the applications of cyclic polypeptides and their prospective applications in the future .
