FOXQ1 gene mediate angiogenesis and proliferation induced by Shh in SW480 cells
10.3969/j.issn.1000-4718.2016.03.014
- VernacularTitle:FOXQ1基因介导了 Shh 诱导的 SW480细胞血管生成及增殖
- Author:
Shuangwei ZHU
;
Xiangshu LI
;
Xudong PENG
;
Cheng CHEN
;
Xiaolong CHEN
;
Zhengqiang WEI
- Publication Type:Journal Article
- Keywords:
FOXQ1 gene;
Sonic hedgehog;
Colorectal cancer;
Cell proliferation;
Angiogenesis
- From:
Chinese Journal of Pathophysiology
2016;32(3):470-476
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the effect of FOXQ1 gene silencing on angiogenesis and proliferation ability of co-lon cancer cells induced by Sonic hedgehog (Shh).METHODS:Lentivirus expressing different FOXQ1-shRNA or nega-tive cantrol (NC)-shRNA was used to infect the SW480 cells.The best silencing condition was screened and used in the following experiments .The SW480 cells were divided into interfered group ( FOXQ1-shRNA) and control group ( NC-shR-NA) .The MTT assay was used to observe the doubling time and cell activity .Tube formation assay was performed to detect the ability of angiogenesis.Meanwhile, the expression of vascular endothelial growth factor (VEGF)-A, matrix metallopro-teinase ( MMP) 2 and cyclin D1 at mRNA and protein levels was determined by real-time PCR and Western blot .After in-duction of the cells by recombinant Shh proteins , the changes of angiogenesis and proliferation ability in each group were detected.At the same time, the transformation of related gene was examined .RESULTS:Compared with control group , the angiogenic ability in interfered group was decreased , and no obvious difference of proliferation ability was observed .The expression of VEGF-A and MMP2 was declined significantly , and the expression of cyclin D 1 was not obviously changed . Recombinant Shh proteins improved the expression of FOXQ1 gene.Compared with NC-shRNA group, after induction, the angiogenic ability of FOXQ1-shRNA group was decreased , and the proliferation ability was not obviously changed .CON-CLUSION:FOXQ1 gene mediates the angiogenic ability but does not affect the proliferation ability of SW 480 cells.Mean-while, it may be regulated by shh pathway .
