Effects of Liuweidihuang pill on insulin levels in sera and pancreatic islets from spontaneous mouse models of human type 2 diabetes administrated with different doses
10.3969/j.issn.1000-484X.2016.03.012
- VernacularTitle:六味地黄丸对自发性2型糖尿病模型小鼠胰岛素表达水平的影响
- Author:
Lu Lü
;
Yuanqiang ZHENG
;
Jingcai ZHANG
;
Siqi LI
;
Xiaodong WANG
;
Xing YIN
;
Jia XU
;
Xinrong HAN
;
Yanchun SHI
- Publication Type:Journal Article
- Keywords:
Liuweidihuang pill;
Spontaneous type 2 diabetes;
Animal model;
Insulin resistance;
Insulin
- From:
Chinese Journal of Immunology
2016;32(3):350-353
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Liuweidihuang pill on the insulin levels in sera and pancreatic islets from spontaneous mouse models of human type 2 diabetes administrated with different doses .Methods:The 6-8 week-old KK-Ay mice were randomly divided into three groups including no drug control group ,low-dose group and high-dose group,in addition C57BL/6J mice were used as a genetic control group .All the animals were given with different dose Liuweidihuang pill solutions or sterile distilled water by intragastrical administration for fifteen weeks .The fasting blood glucose ,body mass and food consumption were measured weekly .The serum insulin levels were surveyed by ELISA .And the insulin levels in the pancreas islets were detected by immunofluorescence and immunohistochemistry .Results:Decreased fasting blood glucose ,controlled body mass and food consumption ,and lower levels of insulin in the sera and pancreas islets were confirmed from the KK-Ay mice administered with Liuweidihuang pill .Furthermore,the low dose program exhibits a stronger effect .Conclusion:Liuweidihuang pill has exhibited relatively therapeutic effects in the spontaneous type 2 diabetes mice including controls of hyperglycemia and body mass and relieving insulin resistance .In addition , the low-dose regimen showed even better treatment in controlling insulin levels in the sera and pancreas islets .
