Development and application of Latex enhanced immune turbidimetry for food allergen
10.3969/j.issn.1000-484X.2016.03.018
- VernacularTitle:基于胶乳增强免疫比浊法的食物过敏原检测试剂盒方法学的建立及临床应用评价
- Author:
Lingling LU
;
Guojun ZHENG
;
Feipei TU
;
Haiou WU
;
Xiaoqiong LIU
- Publication Type:Journal Article
- Keywords:
Latex;
Immunonephelometry;
Methodology;
Allergen
- From:
Chinese Journal of Immunology
2016;32(3):377-381
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method to detect food allergen based on Latex-enhanced immune turbidimetry ( PETIA) and apply to clinic.Methods: The PETIA method was used for evaluating the detection system including standard curve , detection-limit,stability, intra-assay and inter-assay precision and cross-reactivity,clinical normal serum specimens and clinical allergic diseases serum specimens were measured to evaluated the perspective of the clinical application .Results: The standard curve :Y=837.1 x2-125.2x+10.036, linear range: 0.0-400 U/ml, the correlation coefficient of the standard curve was 0.996, the intrabatch and interbatch precision was<10%.The normal reference range was≤33.5 U/ml,the AUC of ROC curve was 0.957,the sensitivity was 89.61%,The specificity was 65.22%, the accuracy was 84.00%, and the positive predicted value was 89.61%, the negative predicted value was 65.22%.the test results have strong correlation with ELISA ( r=0.890 2 ) .Conclusion: The PETIA method for detecting food allergen achieved corresponding clinical application standards and may be used for the diagnosis of food allergies .
