Characterization of naive and memory T cell after immunization with H1N1 influenza vaccine for children
10.3969/j.issn.1000-484X.2016.03.022
- VernacularTitle:儿童新甲型流感疫苗远期特异性CD8＋记忆T细胞的表型特征
- Author:
Junru CHEN
;
Hongbo XIA
;
Xuefen ZHUANG
;
Jinjin GUO
;
Yanli TANG
;
Xinsheng YAO
;
Wanbang SUN
- Publication Type:Journal Article
- Keywords:
Children;
H1N1 vaccine;
Immune vaccine;
Memory T cell;
Immunization long-term;
Immune memory
- From:
Chinese Journal of Immunology
2016;32(3):396-400
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To elucidate the characterization of CD8+T cell in H1N1 influenza vaccine for children.Methods:PBMCs were isolated from 31 children aged from 3 to 6 years old who had accepted H 1N1 influenza vaccine during December 2009 to January 2010.The lymphocytes were joined with the H 1N1 influenza vaccine as experimental group and cultured .The experiment set without vaccine group as control group .At last we detected the surface molecules by FCM .The CCK-8 assay was added to detecting cellular proliferation and cellular proliferation index were detected by CCK-8.Results: CD8+T cells of PBMC in the two groups were 13.41%and 9.41%,P>0.05.CD8+CD45RAA+naive T cells in the two groups were up to more than 80%,P>0.05.The proportion of CD8+CD45ROA+memory T cells in two groups were up to 17%-19%,P>0.05.Two subsets of CD8+CD45ROA+memory T cells :CCR7+and CD62L+single positive memory T cell subsets in the experimental group were significantly lower than that of the control group,P<0.05.The CCK-8 assay was added to detect cellular proliferation .Only 51.16% of which cellular proliferation index was greater than 0.8,with none was greater than 1 in this study.Conclusion:This study showed that the CD4+T cells were low-level,naive T cells (CD8+CD45RAA+)were higher,with antigen stimulation and response.H1N1 vaccination specific memory T cells were few in number , specific memory T cell subsets were diversity , control memory cells were the main phenotypic characteristics .Cellular proliferation index showed that the proliferation of specific CD 8+T cells vaccine was poor .
