Th17 cells adoptive immunotherapy impacts tumor growth of diffuse large B-cell lymphoma-bearing mice
10.3760/cma.j.issn.1009-9921.2015.12.004
- VernacularTitle:Th17细胞过继免疫治疗对荷弥漫大B细胞淋巴瘤小鼠肿瘤生长的影响
- Author:
Xin XU
;
Qingshan LI
;
Guodong ZHU
;
Fang DU
;
Ziyuan ZHAO
- Publication Type:Journal Article
- Keywords:
Lymphoma,large B-cell,diffuse;
Th17 cells;
Immunotherapy,adoptive
- From:
Journal of Leukemia & Lymphoma
2015;24(12):722-725
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impact of Th17 cells adoptive immunity on tumor growth of diffuse large B cell lymphoma (DLBCL)-bearing mice.Methods The CD4+ CD62L+ na(i)ve T cells purified by Mini MACS immunomagnetic beads were stimulated under the condition of TGF-β, IL-6, anti-IFN-γ, anti-IL-4, IL-23.IL-17 expression was detected by ELISA.SCID mice were inoculated with DLBCL cells line SUDHL-4 to estaldish the model of DLBCL-bearing mice, and then they received Th17 cells adoptive immunotherapy at the 0, 8 th or 18th day (3 groups in total, 5 mice pre-group).The tumor volume and the survival of tumor model were observed.Results Formation of tumor nodules in mice was observed at about the 8th day after the mice received the Th17 SUDHL-4 cells.The tumors volume of DLBCL-bearing mice that had received Th17 cells adoptive immunotherapy was obviously smaller than that of the mice had not (P ＜ 0.05).The survival time of the tumor-bearing mice that had received Th17 cells adoptive immunotherapy was also longer than that of the tumor-bearing mice had not (P ＜ 0.05).Conclusion Th17 cells adoptive immunotherapy displays an anti-tumor effect on DLBCL-bearing mice.
