Effect of fluvastatin on the expressions of caspase-12, CHOP and JNK in ischemia-reperfusion brain injury in rats
10.3760/cma.j.issn.1008-1372.2015.11.019
- VernacularTitle:氟伐他汀对大鼠脑缺血再灌注损伤后caspase-12、CHOP及JNK表达的影响
- Author:
Haiying ZHU
;
Yanping CHEN
;
Yingwen MA
- Publication Type:Journal Article
- Keywords:
Pravastatin/PD;
Brain ischemia/DT/ME;
Reperfusion injury/DT/ME;
Caspase 12/ME;
Transcription factor CHOP/ME;
JNK mitogen-activated protein kinases/ME
- From:
Journal of Chinese Physician
2015;17(11):1665-1668
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of fluvastatin on the expressions of caspase-12,CCAAT/enhancer-binding protein homologous protein(CHOP), and c-Jun N-terminal kinases (JNK) in ischemia-reperfusion brain injury in rats.Methods Forty two rats were randomly divided into sham operation group (6 rats), ischemia-reperfusion (I/R) group (18 rats), and fluvastatin (Flu) group (18 rats).The rats of I/R and Flu groups were molded by modified Longa intraluminal thread, then put to death at 2 h occlusion and 24 h reperfusion point.Expressions of caspase-12, CHOP, and JNK were detected with immunohistochemistry and Western blot.Results Immunohistochemistry and Western blot showed that the expressions of caspase-12, CHOP, and JNK were increased at 24 h reperfusion.Compared to I/R group, the expressions of caspase-12 and CHOP in Flu group were decreased significantly (all P ＜0.01);and the expression of JNK had no difference between I/R and Flu groups(P ＞ 0.05).Conclusions The increased expression of caspase-12, CHOP, and JNK showed that endoplasmic reticulum stress was involved in the pathological process of ischemia-reperfusion brain injury.Fluvastatin could inhibit the expression of caspase12 and CHOP, and could delete endoplasmic reticulum stress (ERS) in ischemia-reperfusion brain injury.
