Change of endoplasmic reticulum stress-related molecules in the course of sciatic nerve injury of diabetic rats
10.3760/cma.j.issn.1008-1372.2015.11.016
- VernacularTitle:内质网应激分子在糖尿病大鼠坐骨神经损伤中的变化
- Author:
Wei WANG
;
Shaowei ZHANG
;
Li DING
;
Jing LUO
- Publication Type:Journal Article
- Keywords:
Endoplasmic reticulum;
Stress;
Diabetic neuropathies/ME;
Peripheral nervous system diseases/CO/ME;
Membrane proteins/ME;
Transcription factor CHOP/ME;
Caspase 12/ME
- From:
Journal of Chinese Physician
2015;17(11):1654-1657
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dynamic changes of endoplasmic reticulum stress-related molecules including glucose regulated protein (GRP78), C/EBP homologous protein (CHOP), and caspase-12 in sciatic nerve of diabetic rats and explore its mechanisms.Methods Rats were randomly divided into normal control group (NC) and diabetes mellitus group (DM) that were induced by intraperitoneal injection of Streptozocin after 4 weeks of high-fat chow feeding.Sciatic nerves were isolated for three times at 4 weeks, 8 weeks and 12 weeks after induction of diabetes.The expressions of GRP78, CHOP,and caspase-12 were detected with quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analyses.The morphology of sciatic nerve was investigated with electron microscope.Results With the extension of the course, demyelinating and axonal injury appeared in sciatic nerve of diabetic rats.The expressions of GRP78 mRNA and protein in DM group were significantly higher than NC group at 4 weeks and 8 weeks after induction of diabetes(P ＜0.05, P ＜0.01).The expressions of CHOP mRNA and protein in DM group were significantly higher than NC group at 8 weeks and 12 weeks after induction of diabetes (P ＜ 0.05).The expressions of caspase-12 mRNA and protein in DM group were significantly higher than NC group at 8 weeks after induction of diabetes(P ＜ 0.05, P ＜ 0.01).Conclusions Endoplasmic reticulum stress-related molecules (GRP78, CHOP, and caspase-12) contributed to the peripheral nerve injury of diabetic rats, and displayed dynamic changes.
