Evaluation of the allergenic relationship between Humulus japonicus and Humulus lupulus pollen allergens.
10.4168/aard.2017.5.4.217
- Author:
Chang Gyu JUNG
1
;
Eun Mi YANG
;
Ji Ho LEE
;
Hyun Mi KIM
;
Hae Sim PARK
Author Information
1. Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon, Korea. hspark@ajou.ac.kr
- Publication Type:Original Article
- Keywords:
Cross reactions;
Humulus japonicas;
Humulus lupulus;
Pollen
- MeSH:
Allergens*;
Asthma;
Climate Change;
Cross Reactions;
Desensitization, Immunologic;
Electrophoresis;
Enzyme-Linked Immunosorbent Assay;
Far East;
Gyeonggi-do;
Humans;
Humulus*;
Hypersensitivity;
Immunoglobulin E;
Immunotherapy;
Korea;
Pollen*;
Rhinitis, Allergic;
Sodium
- From:Allergy, Asthma & Respiratory Disease
2017;5(4):217-222
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Humulus japonicus pollen (Hop J) is a major cause of inhalant allergy in autumn of the Far East countries, and its allergenic potency has been increasing with climate changes. Allergen immunotherapy has been considered in Hop J-sensitized allergic patients; however, Hop J allergen extracts for immunotherapy are not commercially available. We speculate that Humulus lupulus pollen (Hop L) belonged to the same genus may share cross-reacting allergens with Hop J and evaluated allergenic relationships between these 2 pollens. METHODS: Thirteen patients with allergic rhinitis and/or asthma sensitive to Hop J pollens were enrolled in Ajou University Hospital, Suwon, Korea. Hop J pollens were collected locally and lyophilized extracts were prepared, while lyophilized Hop L extracts were provided by Lofarma S.p.A. IgE-ELISA/enzyme-linked immunosorbent assay (ELISA) inhibition tests, sodium dodecyl sulphate-polyacrylamide gel electrophoresis and IgE-immunoblot/immunoblot inhibition analysis using sera from the enrolled subjects were performed. RESULTS: All patients had high serum specific IgE to both Hop J and Hop L extracts by ELISA, but no significant correlation was found between these 2 extracts. ELISA inhibition tests showed significant dose-dependent inhibitions on IgE-bindings to Hop L with serial additions of Hop J extracts in a dose-dependent manner, while minimal inhibitions of IgE binding to Hop J were noted with additions of Hop L. IgE-immunoblot analysis demonstrated that the major allergenic component of Hop J at 12 kDa was inhibited by Hop J, while no inhibitions were noted by Hop L extracts on IgE-immunoblot inhibition analysis. CONCLUSION: These findings suggest that there may not be a significant cross-allergenicity between Hop J and Hop L.
