Optimizing the extration progress of ferulie acid and ligustilide form Danggui-Buxue pill by orthogona design

VernacularTitle:正交试验设计优化当归补血丸中阿魏酸和藁本内酯的提取工艺
Author: Hongyan MA ; Xueyan ZHENG ; Haiying JIA ; Xiaoli LI ; Juan LUO
Publication Type:Journal Article
Keywords: Danggui-Buxue pill; Orthogonal test; Ferulic acid; Ligustilide; Chromatography,high pressure liquid
From: International Journal of Traditional Chinese Medicine 2016;38(1):63-66
CountryChina
Language:Chinese
Abstract: Objective To study the optimized process for effective components in Danggui-Buxue pill by orthogona design,and provide the basis for the establishment of quality standard.Methods Ferulie acid and ligustilide were used as examining index,the extraction time,solvent volume and extraction times were used as factors.L9(34) orthogonal test table was used to determine the optimum extraction process.HPLC method was used to establish methodology for ferulie acid and ligustilide.Results According to the result of orthogonal design,comprehensive consider determining the extraction efficiency of ferulic acid and ligustilide the optimal extraction technology as follows:1.0 g Danggui-Buxue pill was extracted by 30 ml of methanol for 60 min,extraction times for 2 times.Conclusions This method is simple with high efficiency.Its HPLC method has high precision and good reproducibility,which can be used for analysis Danggui-Buxue pill.