Effects of Furong-Tongmai capsules on the expressions of interleukin-1 and tumor necrosis factor-α in the sciatic nerve in diabetic rats
10.3760/cma.j.issn.1673-4246.2016.01.014
- VernacularTitle:复荣通脉胶囊对糖尿病模型大鼠坐骨神经白细胞介素-1及肿瘤坏死因子-α表达的影响
- Author:
Lixin WANG
;
Yuansong WANG
;
Fengsheng TIAN
;
Ronggang CUI
;
Yun BIAN
;
Caixia JIA
;
Qingkai WANG
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus,experimental;
Diabetic neuropathies;
Drugs,Chinese herbal;
Sciatic nerve;
Interleukin-1;
Tumor necrosis factor-or;
Rats;
Furong-Tongmai capsules
- From:
International Journal of Traditional Chinese Medicine
2016;38(1):53-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Furong-Tongmai capsules on the expressions of interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) in the sciatic nerve in diabetic rats.Methods A total of 50 rats were randomly divided into the normal control group,model group,and low-,medium-,and high-dose of Furong-Tongmai groups,10 rats in each group.Diabetes mellitus in rats were induced by intraperitoneal streptozotocin injection (60 mg/kg).The rats in the low-,medium-,and high-dose of Furong-Tongmai groups were intragastric administrated with Furong-Tongmai suspension 0.7,1.4 and 2.8 g/kg daily for 8 weeks,respectively.The rots in the normal control group and model group were intragastric administrated with equal-volume normal saline daily for 8 weeks.The expression levels of IL-1 and TNF-α in the sciatic nerve were detected with immunohistochemistry staining.Results The expression levels of IL-1 (1.43% ± 0.17% vs.0.21% ± 0.09%;P<0.05) and TNF-α (1.98% ± 0.12% vs.0.35% ± 0.03%;P<0.05) in the model group were significantly increased than those in the normal control group.The expression levels of IL-1 (0.54% ± 0.14%,0.51% ± 0.13% vs.1.43% ± 0.17%;all P<0.05) and TNF-α (0.57% ± 0.17%,0.49% ± 0.15% vs.1.98% ± 0.12%;all P<0.05) in the medium-,and high-dose of Furong-Tongmai groups were significantly decreased than those in the model group and low-dose of Furong-Tongmai group (1.08% ± 0.18% in IL-1,1.11% ± 0.09% in TNF-α;all P<0.05).Conclusion Furong-Tongmai capsules can reduce the expressions of IL-1 and TNF-α in sciatic nerve in diabetic rats.