The effects of IL-1 beta on intestinal epithelial barrier permeability
10.3760/cma.j.issn.1673-4408.2016.05.015
- VernacularTitle:IL-1β对肠上皮细胞屏障通透性的影响
- Author:
Nan LIN
;
Xu TENG
;
Lingfen XU
;
Mei SUN
- Publication Type:Journal Article
- Keywords:
Intestinal epithelial burrier;
Transepithelial electrical resistance;
Interleukin-1β;
Inflammatory bowel disease
- From:
International Journal of Pediatrics
2016;43(5):409-412
- CountryChina
- Language:Chinese
-
Abstract:
Objective Inflammatory bowel disease is an important chronic gastrointestinal disease of childhood and adolescence.Intestinal mucosa barrier damage plays an important role in its pathogenesis.This study attempts to use IL-1β stimulating Caco-2 cell monolayer simulates inflammatory intestinal epithelial barrier in vitro,provides the basis for studying the pathogenesis and treatment of IBD.Methods Caco-2 cells were cultivated in vitro until the 21st day to simulate intestinal epithelial cell monolayer barrier.The cells of inflammation group one were disposed with IL-1β for 2 h since the 5th day and detected TEER every other day until the 21st day.The cells of inflammation group two were disposed with IL-1 β at the 18th day for 0,12,24,48,72h, and detected TEER respectively.Normal control group cells were cultured with common medium and detected TEER at the corresponding time point.Results The TEER of Caco-2 cells gradually increased from the 5th to 15th days,reached 600Ω·cm2 in the 15th day of a plateau until to the 21st day.Since the 5th day,the TEER of inflammation group one were all lower than normal group,and still to the 21st day ＜ 500Ω·cm2.Inflammation group two shows the time dependence TEER gradually reduce,peaked at 48 hours,then a slight increase in 72 hours.Conclusion The Caco-2 cells cultured for 2 ～ 3 weeks can form intestinal epithelial monolayer barrier with polarity,then treated with IL-1 β can manufacture inflammatory intestinal epithelial barrier model in vitro.
