Expression and significance of Oct4 B1 in colorectal cancer stem cells
10.3969/j.issn.1000-484X.2016.06.005
- VernacularTitle:Oct4 B1在结直肠癌干细胞中的表达及意义
- Author:
Jiaping CHENG
;
Jiang LI
;
Xian SU
;
Yilin CHEN
;
Qingliang ZENG
;
Kunming WEN
- Publication Type:Journal Article
- Keywords:
Colorectal cancer;
Cancer stem cells;
Oct4;
Oct4B1
- From:
Chinese Journal of Immunology
2016;32(6):794-797
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression and its possible role of Oct4B1 subtype of Embryonic stem cell transcription factor Oct4 in colorectal cancer stem cells. Methods: 3D microspheres were cultured by suspension culture to human colorectal cancer cell line SW480 cells. The 3D microspheres and SW480 cells were used as the research objects. Whether 3D microspheres were enriched cancer stem cells,we used the methods of cell differentiation experiments,soft agar cloning experiments,and the expression levels of cancer stem cells markers CD133,CD44 detected by flow cytometry. The expression levels of Oct4B1 mRNA were detected by RT-qPCR. Results:3D microspheres could differentiate into normal cancer cells. Compared with the parental SW480 cells,in vitro colony formation was significantly enhanced(P<0. 01),the percentage of positive cells of CD133 and CD44 were significantly increased ( P < 0. 01 ), the expression levels of Oct4B1 mRNA were obviously higher ( P < 0. 01 ) in 3D microspheres. Conclusion: Oct4B1 subtype of Embryonic stem cell transcription factor Oct4 in 3D microspheres enriched human colorectal cancer stem cells,which may be involved in the regulation of colorectal cancer stem cells.
