Identification of a new shrimp allergen enolase from Litopenaeus vannamei
10.3969/j.issn.1000-484X.2016.06.008
- VernacularTitle:凡纳滨对虾新过敏原烯醇化酶的鉴定
- Author:
Siyi TAN
;
Jianfang HUANG
;
Yifan SUN
;
Chengbin GUO
;
Junjian XIANG
- Publication Type:Journal Article
- Keywords:
Litopenaeus vannamei;
Allergen;
Enolase;
Mass spectrometry;
Western blot
- From:
Chinese Journal of Immunology
2016;32(6):808-811
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify enolase,47 kD allergen,from Litopenaeus vannamei by Mass spectrometry. Methods: The proteins were extracted from Litopenaeus vannamei tissue with acetone precipitation method. The protein components were analyzed by SDS-PAGE and Western blot. By using Matrix-Assisted Laser Desorption/Ionization time of flight mass spectrometry ( MALDI-TOF/TOF-MS) ,the 47 kD allergen from Litopenaeus vannamei was identified as enolase. Results:By SDS-PAGE,we proved that the native protein components from Litopenaeus vannamei were completely. According to the Western blot result more than 14 components could react with the positive serum. MALDI-TOF/TOF analysis results showed that the suspected proteins were enolase. A sensitization frequency was 55%. Conclusion:Enolase was identified as a new allergen of Litopenaeus vannamei.
