Effect of GRP94 silencing on proliferation and apoptosis of human breast carcinoma MCF7 cells

VernacularTitle:沉默GRP94基因对乳腺癌MCF7细胞增殖和凋亡的影响
Author: Jianjun FAN ; Jiayan WU ; Yun LI ; Fan ZENG ; Fangzhou SONG
Publication Type:Journal Article
Keywords: GRP94 gene; MCF7 cells; Proliferation; Apoptosis
From: Chinese Journal of Immunology 2016;32(6):828-831
CountryChina
Language:Chinese
Abstract: Objective:To determine the effects of silencing glucose regulated protein ( GRP94 ) on the proliferation and apoptosis of breast carcinoma MCF7 cells. Methods:Chemically synthesized siRNA targeting GRP94 gene and transfected into MCF7 cells used by Liopfectamine RNAIMAX. The mRNA and protein expression levels of GRP94,cyclinD1,Bax and Bcl-2 were detected by Real-time PCR and Western blot. CCK8 assay was used to detect the effect of specific GRP94 siRNA on cell proliferation and the effect on cell cycle and apoptosis were analyzed by flow cytometry and Hoechst 33258 staining. Results:Compared with the siRNA-NC cells, the expression of GRP94 was significantly down-regulated in MCF7 cells. Knockdown of GRP94 in MCF7 cells decreased cell proliferation and promoted cell apoptosis. The expression of cyclinD1and Bcl-2levels were significantly reduced, and Bax level was increased in siRNA-GRP94 MCF7 cells. Conclusion: The siRNA-mediated GRP94 silence significantly inhibits MCF7 cell proliferation,promoted cell apoptosis by down-regulating cyclin D1,Bcl-2 expression and up-regulating the Bax expression in MCF7 cells.