The effect of Er:YAG laser radiation on the proliferation of human periodontal ligament fibroblasts
10.3969/j.issn.1001-3733.2016.03.013
- VernacularTitle:Er：YAG 激光照射对人牙周膜成纤维细胞增殖的影响
- Author:
Zhixiong ZHOU
;
Sun ZHANG
- Publication Type:Journal Article
- Keywords:
Er:YAG laser;
Human periodontal ligament fibroblast;
Cell proliferation
- From:
Journal of Practical Stomatology
2016;32(3):358-361
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Er:YAG laser on the proliferation of human periodontal ligament fibroblasts(hP-DLFs).Methods:Human periodontal ligament fibroblasts were cultured in vitro and identified by immunohistochemistry.The cells of 5th passage were divided into 5 groups.The cells in group A without treatment were used as the controls,in group B,C,D and E were treated with Er:YAG laser of 10 Hz at 50 mJ,100 mJ,150 mJ and E-200 mJ for 1 s respectively.The proliferation of the cells was examined on day 1,3,5,7,9 by CCK-8.Results:The proliferation of hPDLFs in group B,C,D and E increased more than that in group A(P <0.05)5 d after Er:YAG laser radiation.Conclusion:Low intensity of Er:YAG laser radiation can promote the prolifera-tion of human periodontal ligament fibroblasts.
