Establishment of chemiluminescence immunoassay for AngiotensinΙ
10.3969/j.issn.1000-484X.2016.06.015
- VernacularTitle:血管紧张素Ι化学发光免疫分析方法的建立
- Author:
Chaoyi YANG
;
Tingting FENG
;
Xuefeng ZHANG
;
Jinhui LU
;
Bin WANG
- Publication Type:Journal Article
- Keywords:
AngiotensinⅠ;
Biotin;
Fluorescein;
Streptavidin-enzyme;
Chemiluminescence immunoassay
- From:
Chinese Journal of Immunology
2016;32(6):838-841
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The chemiluminescence immunoassay for AngiotensinⅠ ( AngⅠ ) was developed by the competition method. The renin activity was calculated by the determination of Ang Ⅰ. Methods: AngⅠ antigen was labeled with biotin and fluorescein labeled AngⅠantibody,and anti fluorescein antibody was used to coated with the microporous plate . Results:The standard range of the method was 100-7 800 pg/ml. The assay sensitivity was 24. 3 pg/ml. The intra- and inter-assay coefficients of variance were 5. 6%-8. 7% and 6. 8%-10. 4% respectively. Analytical recovery was 95. 4%-105. 3%. The correlation coefficients between measured and expected values were 0. 999 after serial diluted. Compared with radioimmunoassay kit,the correlative equation was y=0. 95x+235. 70,correlation coefficient was 0. 973. Coated microporous plate and biotin labeled Ang Ⅰ antigen,and fluorescein labeled Ang Ⅰantibody at 37℃ for a week with good stability. Conclusion: The results of the method were in accord with the basic requirements of immunoassay.
