The inhibitory effects of chlorhexidine in the development of rat periodontitis models
10.3969/j.issn.1001-3733.2016.03.001
- VernacularTitle:氯已定在大鼠牙周炎模型建立过程中的抑制作用
- Author:
Hongyan WANG
;
Lisi TAN
;
Chunliang MA
;
Shuo GUAN
;
Yaping PAN
- Publication Type:Journal Article
- Keywords:
Chlorhexidine;
Rats;
Periodontitis models
- From:
Journal of Practical Stomatology
2016;32(3):303-307
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effects of different concentrations of chlorhexidine in the development of peri-odontitis models in rats.Methods:periodontitis models were established by the ligation of bilateral first molars and orally challenge with P.gingivalis W83.0.05%,0.1%,0.2% and 0.5% chlorhexidine were used to wash the periodontal pocket and oral mucosa of the rats.4 weeks later,absolute real time quantitative PCR was used to count the copy of P.gingivalis W83 in rat periodontal pockets.Scanning electron microscopy was used to observe the distribution of P.gingivalis W83 on rat teeth surface.Immunohisto-chemical technique was used to detect the expression of TNF-αin gingival tissue of the rats.Results:0.2% and 0.5% chlorhexi-dine reduced the copy of P.gingivalis W83 on teeth surface and in periodontal pockets (P <0.05);0.1% -0.5% chlorhexidine reduced the expression of TNF-αin gingival tissue (P <0.05).Conclusion:0.1% -0.2% chlorhexidine can inhibit the develop-ment of chronic periodontitis in rats.
