The effects of brushite coating extract with strontium on the osteoblast-related factor expression of MC3T3-E1 cells
10.3969/j.issn.1001-3733.2016.03.006
- VernacularTitle:透钙磷石涂层浸提液掺锶对 MC3T3-E1细胞成骨相关因子表达的影响
- Author:
Chenjuan WEN
;
Yongqiang LIANG
;
Dongying HAN
- Publication Type:Journal Article
- Keywords:
Coating;
Strontium;
Brushite;
Osteoblasts
- From:
Journal of Practical Stomatology
2016;32(3):325-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of brushite coating with strontium on the osteoblast-related factor expression of MC3T3-E1 cells.Methods:Brushite coating on tinanium surface was prepared by electrochemical deposition and the extract of the coating with stronium at 0.1%,0.5% and 1% were prepared respectively.MC3T3-E1 cells were cultured with the extracts,cell prolifera-tion,ALP activity,bFGF and VEGF mRNA were examined by MTT assay,ALP staining and RT-PCR respectively.Results:Stronti-um containing extracts promoted the proliferation,increased the ALP activity and mRNA expression of bFGF and VEGF(P <0.05)of MC3T3 cells.Conclusion:Brushite extract containing strontium may promote osteogenic function of MC3T3-E1 cells.
