Biological characteristics of stem cells from deciduous tooth pulp of Beagle dog
10.3969/j.issn.1001-3733.2016.03.007
- VernacularTitle:犬乳牙牙髓干细胞体外培养及生物学性能研究
- Author:
Fei LIU
;
Yang LIU
;
Shanshan DAI
;
Ninghu MA
;
Lin ZHANG
;
Qingyu GUO
- Publication Type:Journal Article
- Keywords:
Canine;
Deciduous tooth pulp stem cells(DTPSCs);
Multilineage differentiation
- From:
Journal of Practical Stomatology
2016;32(3):331-335
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To culture and characterize the deciduous tooth pulp stem cells(DTPSCs)of Beagle dog.Methods:DTPSCs were cultured using enzyme tissue block method from Beagle dog aged 6 weeks.The cells were purified by cloning culture,identified by immunohistochemistry and flowcytometry.The biological characteristics were studied by CCK-8 assay,osteogenetic induction,li-pogenic induction and dentinogenic induction assays.Results:Beagle stem cells from deciduous tooth pulp were obtained,the cell colony formation rate was 32%.The cells were STRO-1 and CD146 positive,CD14,CD45 and CD86 negative.After multiple induc-tion culture the cells were positive for alizarin red staining,oil red staining,ALP expression and DSSPP expression.Conclusion:The deciduous tooth pulp stem cells of Beagle dog have multilineage differentiation abilities.
