The effects of lactoferrin on the proliferation,migration and osteogenic differentiation of human periodontal ligament cells
10.3969/j.issn.1001-3733.2016.03.023
- VernacularTitle:乳铁蛋白对人牙周膜细胞增殖迁移分化的影响
- Author:
Bingyu JIN
;
Yanxiang XUE
;
Ying LIU
;
Jie GAO
- Publication Type:Journal Article
- Keywords:
Lactoferrin(LF);
Human periodontal ligament cells(HPDLCs);
Osteogenic differentiation
- From:
Journal of Practical Stomatology
2016;32(3):406-411
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of lactoferrin(LF)on the proliferation,migration and osteogenic differentiation of human periodontal ligament cells(HPDLCs)in vitro.Methods:HPDLCs were cultured and identified.The proliferation and migration of HP-DLCs cultured with 0,10 and 20 μg/ml lactoferrin respectively were tested by MTT assay,Transwell assay and scratch test.The os-teogenic differentiation of the cells was evaluated using alizarin red staining and real-time PCR.Results:Lactoferrin at 10 and 20μg/ml increased the proliferation(P <0.05),increased the quantities of mineralized nodules and the expression of alkaline phospha-tase(ALP),osteocalcin(OCN)and osteopontin(OPN)(P <0.05).Conclusion:Lactoferrin promotes the proliferation,migration and osteogenic differentiation of HPDLCs.
