Regulation of energy metabolism in colon cancer cells by chidamide
10.3867/j.issn.1000-3002.2016.05.009
- VernacularTitle:西达本胺对结肠癌细胞能量代谢的调节作用
- Author:
Mu HE
;
Zhixin QIAO
;
Suping REN
;
Changlan LI
;
Yanbing WANG
;
Qiyuan GUI
;
Yu WANG
;
Yunjing LUO
;
Qun YU
- Publication Type:Journal Article
- Keywords:
colon cancer cells;
chidamide;
lactate dehydrogenase A;
Warburg effect
- From:
Chinese Journal of Pharmacology and Toxicology
2016;30(5):539-544
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To observe the regulation effect of chidamide on energy metabolism in HCT-8 and HT-29 cells. METHODS HCT-8 and HT-29 cells were treated with chidamide 5,10 and 20 μmol · L-1. Morphological changes of these cells were observed under an ordinary optical microscope. Cell proliferation was detected by MTT. ATP production was determined by CellTiter-Glo? assay kit. Metabolic changes were tested by glycolytic stress kit. The mRNA level of lactate dehydrogenase A (LDH-A)was analyzed by real-time quantitative PCR,whereas the protein level of LDH-A was analyzed by Western blotting. RESULTS Compared with control group,cell morphology of HCT-8 and HT-29 cells in chidamide treated group was irregular,accompanied by deformation,shrinkage and cell debris, and the inhibitory rate of proliferation increased(P<0.05). There was no significant difference in ATP total content between chidamide 5 and 10 μmol · L-1 16 h treatment groups,but in chidamide 20 μmol · L-1 treatment group it was decreased(P<0.05). Chidamide 20μmol · L-1 had no effect on oxygen consumption rate, but glycolysis ATP generation rate was reduced by 30.7% and 37.9%(P<0.05),respectively. Chidamide 20μmol · L-1 had no effect on LDH-A mRNA level,but it decreased the protein level of LDH-A(P<0.01). CONCLUSION Chidamide can abate the respiratory metabolic ability of HCT-8 and HT-29 cells. The mechanism may be related to the down-regulation of LDH-A.
