A calcified biomimetic scaffold for bone tissue engineering in the treatment of martialarts-induced ankle injury
10.3969/j.issn.2095-4344.2016.21.004
- VernacularTitle:含钙化层仿生组织工程骨支架对运动性踝关节损伤的修复
- Author:
Huipeng CHEN
;
Lingna LIU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(21):3065-3070
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:In sports or in daily life, damage due to sudden power, especialy due to non-physiological release,is commonly seen. For example, during basketbal, soccer, rugby, or martial arts movement, oppositional and explosive movements result in a higher incidence of ankle injuries. While conventional methods can improvesymptoms, the long-term efficacy is unsatisfactory, accompanied by a higher incidence of complications that are likely to cause secondary damage.
OBJECTIVE:To prepare a calcified biomimetic scaffold for bone tissue engineering and to observe the therapeutic effect of this scaffold onmartialarts-induced ankle injuries.
METHODS:Eighty patients withmartialarts-induced ankle injury were selected from Chengdu Sport Institute between December 2014 and December 2015. These patients were randomly assigned to control group with drug treatment and biomimetic scaffold group with calcified biomimetic scaffold implantation (n=40 per group). Acelular suspension prepared by goat cartilage was used to make cartilage tissue blocks with a calcified layer with a diameter of 8 mm in a prechiled abrasive apparatus. Then, the calcified biomimetic scaffold for bone tissue engineering was prepared using lyophilization and chemical crosslinking methods.
RESULTS AND CONCLUSION:Osteochondral tissues were partialy hyalinized on the surface with the presence of osteochondral calcified layer. The hyalinized cartilage was white in color, the calcified layer existed between normal osteochondral tissues, and the subchondral bone was considered as the cancelous bone. Then the calcified layer was stained using hematoxylin-eosin. We found that cartilage cels in the calcified layer were basicaly removed, forming “empty nests” one by one. But the structure of bone cartilage in the tissue block, the calcified layer and the subchondral bone retained wel. For pain assessment, visual analog scale scores were detected and showed no difference between two groups prior to treatment (P> 0.05), but became significantly higher in the biomimetic scaffold than the control group at 1 and 4 weeks after treatment (P< 0.05). Besides, the biomimetic scaffold exhibited better therapeutic efficacy than the drug treatment (P< 0.05). Overal, this study successfuly prepare the calcified biomimetic scaffold for bone tissue engineering that is suitablefor repair of sport-induced ankle injuries.
