Mycobacterium avium-derived lipids attenuate immune response against Mycobacterium in human alveolar macrophages
10.3969/j.issn.1673-4130.2016.09.006
- VernacularTitle:鸟分枝杆菌脂类诱导的人肺泡巨噬细胞抗结核免疫反应性的改变及机制的初步研究
- Author:
Honghu PENG
;
Liping SHI
;
Kangshi ZHOU
;
Daqing YANG
- Publication Type:Journal Article
- Keywords:
Mycobacterium avium-derived lipids;
human alveolar macrophages;
immunity function
- From:
International Journal of Laboratory Medicine
2016;37(9):1172-1174
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of exposure to MALs on immunity against Mycobacterium tuberculosis in human alveolar macrophages .Methods Plate counting were used to evaluate the effect of resisting Mycobacterium tuberculosis in human alveolar macrophages .Culture supernatants were harvested and human TNF‐α was measured by ELISA kit .Total nitrite levels in the media were measured using the Griess reagent .Results MALs stimulation attenuated the control of intracellular mycobacterial growth in human alveolar macrophages .The production of proinflammatory cytokines TNF‐α and Nitric oxide in human alveolar macrophages induced by Mycobacterium tuberculosis purified protein derivatives tuberculin (Mt‐PPD) and human interferonγwere attenuated by pretreatment in vitro with MALs .Conclusion MALs attenuated the responses of human alveolar macrophages a‐gainst Mycobacterium tuberculosis ,which may provide the new evidence to explain the phenomena in which some component of Mycobacteria in environment impacts the immunity against Mycobacterium tuberculosis infection .
