Stabilizing Microtubular Network Facilitates the Intracellular Growth of Orientia tsutsugamushi.
- Author:
Mee Kyung KIM
1
;
Mi Jeong KIM
;
Byung Uk LIM
;
Jae Seung KANG
Author Information
- Publication Type:Original Article
- Keywords: Orientia tsutsugamushi; Microtubule; Taxol; Daunomycin; Colchicine
- MeSH: Colchicine; Cytoplasm; Daunorubicin; Microtubule-Organizing Center; Microtubules; Orientia tsutsugamushi*; Paclitaxel
- From:Journal of Bacteriology and Virology 2006;36(2):51-57
- CountryRepublic of Korea
- Language:English
- Abstract: Microtubule network provides many intracellular microbes with an efficient way to move within host cells. Orientia tsutsugamushi move from the cell periphery to the microtubule organizing center (MTOC) by dynein-dependent mechanism. In this study, we investigated the role of microtubule on the growth of O. tsutsugamushi. The treatment of infected cells with taxol as well as daunomycin enhanced the bacterial growth in contrast to colchicine. Immunofluorescent (IF) staining of taxol-treated cells exhibited that O. tsutsugamushi clustered tightly near the nucleus with thick bundles of microtubules, whereas dispersed in the cytoplasm in colchicine-treated cells. These results suggest that microtubule network facilitate the growth of O. tsutsugamushi.
