Effects of combined treatment using umbilical cord blood mesenchymal stem cell transplantation and paclitaxel on proliferation, apoptosis, and invasion of A2780 ovarian cancer cells
10.3969/j.issn.2095-4344.2016.20.006
- VernacularTitle:脐血间充质干细胞移植联合紫杉醇对卵巢癌A2780细胞增殖、凋亡及侵袭的影响
- Author:
Fengzhen CHEN
;
Lirong YIN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(20):2927-2932
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:An intensivestudy on ovarian cancer A2780 cels contributes to modify treatment strategies targeting ovarian cancer stem cels and enhance survival rate of ovarian cancer patients.
OBJECTIVE:To investigate the effects of umbilical cord blood mesenchymal stem cels (UC-MSCs) transplantation combined with paclitaxel on proliferation, apoptosis, and invasion of A2780 human ovarian cancer celsin vitro.
METHODS:A2780 human ovarian cancer cels were assigned into three groups: blank control group (routine cel culture),UC-MSCs group (addition of suspension containing 1×106UC-MSCs) and combined treatment group (combined addition of suspension containing 1×106UC-MSCs and 250μL of 1μmol/L paclitaxel solution). CD133+antigen expression, proliferation, apoptosis, and invasion were determined by immunofluorescent staining, flow cytometry, TUNEL staining, and Transwel chamber invasion assay, respectively.
RESULTS AND CONCLUSON:CD133+antigen expression intheUC-MSCs group and combined treatment group was significantly decreased compared withtheblank control group (P< 0.05). Proliferation and invasion abilities were significantly decreased, while apoptosis cel number was significantly increased inthecombined treatment group compared with the other two groups (P< 0.05). These findings indicate that the combined treatment of UC-MSCs transplantation and paclitaxel can inhibit proliferation and invasion and promote apoptosisof A2780 human ovarian cancer cels.
