Variation of stromal cell-derived factor-1 level in a limbal stem cell deficiency model
10.3969/j.issn.2095-4344.2016.19.016
- VernacularTitle:角膜缘干细胞缺乏是否可导致归巢因子基质细胞衍生因子1水平变化
- Author:
Wei ZHANG
;
Wenzhi HUANG
;
Yuping ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(19):2844-2849
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:How to promote homing of scarce limbal stem cel s is the key to improving transplantation efficiency. And stromal cel-derived factor-1 plays an important role in the repair of retinal pigment epithelial cel s injury. OBJECTIVE:To observe changes of stromal cel-derived factor-1 levels in rat and mouse limbal stem cel deficiency models in order to confirm whether these changes wil induce peripheral blood stem cel s homing into the cornea. METHODS:Eye models of limbal stem cel deficiency were prepared in rats and mice by cutting method, then peripheral blood samples were continuously drawn to detect the mass concentration of stromal cel-derived factor-1 by enzyme-linked immunosorbent assay within 1 month after modeling. RESULTS AND CONCLUSION:In the rat peripheral blood, the mass concentration of stromal cel-derived factor-1 started to increase at 24 hours after modeling and firstly peaked at the 3rd day;then it tended to decrease and reached a second peak lower than the first one at the 25th day;subsequently, it returned to the normal level. In the meanwhile, the mass concentration of stromal cel-derived factor-1 in the mouse peripheral blood, increased at 12 hours after modeling, peaked at the 3rd day, subsequently decreased until another peak lower than the former one presented at the 20th day, and final y it returned to the normal level. In conclusion, the level of stromal cel-derived factor-1 in the peripheral blood is significantly associated with limbal stem cel s deficiency, which may play an important role in corneal injury repair.
