Expression of lncRNA PCGEM1 and AR co-localization in prostate cancer and tis signiifcance
10.3969/j.issn.1007-3969.2016.04.006
- VernacularTitle:LncRNA PCGEM1和雄激素受体在前列腺癌中的共定位表达及临床意义
- Author:
Zhuxian ZHU
;
Chen YU
;
Zhongmin QIU
;
Hanjing LV
;
Guangjv GUAN
;
Ziqiang ZHANG
- Publication Type:Journal Article
- Keywords:
Long non-coding RNA;
Androgen receptor;
Prostate cancer
- From:China Oncology
2016;26(4):320-325
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:Long non-coding RNA (lncRNA) could be an important player in cancer biology. Recent studies showed that lncRNA PCGEM1 might be important in the regulation of androgen recep-tor (AR) signaling pathway. We tried to observe the expressions of lncRNA PCGEM1 and AR in prostate cancer, and investigate their role and signiifcance in prostate cancer genesis and progress.Methods:The expression of lncRNA PCGEM1 was observed in prostate cancer by lfuorescencein situ hybridization (FISH) technique. Then detection of AR was performed by immunolfuorescence histochemistry methods. Their co-effective role was checked by RNA pull-down technique.Results:Compared with the AR-independent cell line such as PC3 or DU145, AR-dependent cell line such as LNCaP showed much higher expression of lncRNA PCGEM1 (P<0.01). PCGEM1 and AR could be co-localized in most of these prostate cancer samples, especially in the metastasis samples. Moreover, androgen deprivation promoted the translocation of PCGEM1 into nucleus. RNA pull-down results also proved the co-effective role of PCGEM1 and AR.Conclusion:This study showed that lncRNA PCGEM1 was highly expressed in metastatic prostate cancer. It was related to the progress and malignant behavior of the prostate cancer. Its co-localization with AR may play an important role in prostate cancer genesis and progress.
