Urinary metabolic profiling research on the protective function of Schisandra chinensis against glomerulonephritis of rat models
10.3760/cma.j.issn.1673-4246.2016.06.013
- VernacularTitle:五味子水提液治疗肾小球肾炎大鼠的尿液代谢组学研究
- Author:
Jiatian WANG
;
Hui XIONG
;
Ning ZHANG
;
Bin LIU
;
Jianmin LI
;
Fang GENG
- Publication Type:Journal Article
- Keywords:
Schisandra chinensis;
Gas chromatography-mass spectrometry;
Metabonomics;
Glomerulonephritis;
Biomarkers
- From:
International Journal of Traditional Chinese Medicine
2016;38(6):531-534
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Schisandra chinensis on the treatment of glomerular nephritis in rats by gas chromatography mass spectrometry (GC-MS).Methods The rats were randomly divided into normal group, model group, and the treatment group, 10 rats in each group. We employed the C-BSA injuction to induce situ immune complexes nephritis rate model. Then 24 hour urinary protein was detected after 12 days and a urine metabonomic technology combined with pattern recognition method was used to identify the network analysis, which focuses on the key metablites and enzmys with glomerulonephritis disease.Results After 12 days, compared with the control group, the 24 h urinary protein content (9.24 ± 2.69 mg vs. 76.04 ± 18.88 mg) significantly decreased (P<0.01). Urine metabonomicsi dentified 15 endogenous biomarkers, which included unsaturated fatty acid biosynthesis, pyruvate metabolism and glycolysis metabolism. These metabolic pathways are associated with kidney diseases.Conclusion The effect of Schisandra chinensis on glomerular nephritis may treated by regulating the metabolism of arachidonic acid and fatty acid in four.
