Anti-proliferative Effect of TanshinoneⅡA on Cervical Squamous Cancer Cells and Its Estrogen Receptor Subtype Mediated Mechanism
10.3969/j.issn.1005-5304.2016.06.014
- VernacularTitle:丹参酮ⅡA抗宫颈癌鳞癌细胞增殖效应及其雌激素受体亚型介导机制的研究
- Author:
Jinfeng ZANG
;
Piwen ZHAO
;
Junyun ZHAO
;
Shiying TAO
;
Liping SUN
;
Jianzhao NIU
- Publication Type:Journal Article
- Keywords:
TanshinoneⅡA;
cervical squamous cancer Siha cell;
estrogen receptor;
cell proliferation;
cell cycle;
phospho-extracellular regulate kinase;
Cyclin D
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(6):51-55
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the effects of tanshinoneⅡA on proliferation of cervical squamous cancer Siha cells; To discuss its possible molecular mechanism.Methods Cervical squamous cancer Siha cells were treated with different doses of tanshinoneⅡA. The effects of tanshinoneⅡA on proliferation of Siha cells were measured by MTT assay and flow cytometry analysis. The effects of tanshinoneⅡA on expression levels of phospho-extracellular regulate kinase (p-ERK) and Cyclin D in Siha cells were measured by Western blot.Results 1×10-5, 5×10-6, 1×10-6, 5×10-7 mol/L tanshinoneⅡA significantly inhibited Siha cell proliferation and such effect could be enhanced by ERα antagonist MPP and attenuated by ERβ antagonist PHTPP. 1×10-5, 5×10-6, 1×10-6 tanshinoneⅡA could significantly decrease the proliferation index of Siha cells. 1×10-5, 5×10-6, 1×10-6, 5×10-7 mol/L tanshinoneⅡA could significantly reduce the protein expression levels of p-ERK and Cyclin D of Siha cells.ConclusionTanshinoneⅡA can inhibit cervical squamous cancer Siha cell proliferation and such effect is realized via estrogen receptor pathway. TanshinoneⅡA plays anti-proliferation roles by reducing the expression levels of p-ERK and Cyclin D.
